GATE 2025 syllabus for Biochemistry (XL-Q) gives you details of the latest GATE syllabus for the subject released by the official gate organizing institute IIT Roorkee 2025. We also created an easy-to-use ad-free mobile app for the GATE syllabus, previous year papers with keys, gate calculator, virtual calculator, and more. Download iStudy App for all GATE preparation needs.
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GATE 2025 Syllabus – Biochemistry (XL-Q)
Section 1
Organization of life
Importance of water; Structure and function of biomolecules: Amino acids, Carbohydrates, Lipids, Proteins and Nucleic acids; Protein structure, folding / misfolding and function; Myoglobin, Hemoglobin, Lysozyme, Ribonuclease A, Carboxypeptidase and Chymotrypsin.
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Section 2
Enzyme kinetics, regulation and inhibition
Vitamins and Coenzymes; Bioenergetics and metabolism; Generation and utilization of ATP; Metabolic pathways and their regulation: glycolysis, TCA cycle, pentose phosphate pathway, oxidative phosphorylation, gluconeogenesis, glycogen and fatty acid metabolism; Metabolism of Nitrogen containing compounds: nitrogen fixation, amino acids and nucleotides. Photosynthesis, Calvin cycle.
Section 3
Biochemical separation techniques
ion exchange, size exclusion and affinity chromatography, centrifugation; Characterization of biomolecules by electrophoresis; DNA- protein and protein – protein interactions; UV-visible and fluorescence spectroscopy; Mass spectrometry.
Section 4
Cell structure and organelles
Biological membranes; Action potential; Transport across membranes; Membrane assembly and Protein targeting; Signal transduction; Receptor-ligand interaction; Hormones and neurotransmitters.
Section 5
DNA replication, transcription and translation
DNA damage and repair; Biochemical regulation of gene expression; Recombinant DNA technology and applications: PCR, site directed mutagenesis, DNA-microarray; Next generation sequencing; Gene silencing and editing.
Section 6
Immune system
Innate and adaptive; Cell of the immune system; Active and passive immunity; Complement system; Antibody structure, function and diversity; B cell and T Cell receptors; B cell and T cell activation; Major histocompatibilty complex; Immunological techniques: Immunodiffusion, immune-electrophoresis, RIA and ELISA, flow cytometry; monoclonal antibodies and their applications.
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GENERAL APTITUDE (Common for all branches)
Verbal Aptitude
Basic English Grammar
Tenses, articles, adjectives, prepositions, conjunctions, verb-noun agreement, and other parts of speech
Basic Vocabulary
Words, idioms, and phrases in context
Reading and Comprehension
Narrative Sequencing
Quantitative Aptitude
Data Interpretation
Data graphs (bar graphs, pie charts, and other graphs representing data), 2- and 3-dimensional plots, maps, and tables
Numerical Computation and Estimation
Ratios, percentages, powers, exponents and logarithms, permutations and combinations, and series
Mensuration and Geometry
Elementary Statistics and Probability
Analytical Aptitude
Logic
Deduction and Induction
Analogy
Numerical Relations and Reasoning
Spatial Aptitude
Transformation of Shapes
Translation, rotation, scaling, mirroring, assembling, and grouping
Paper folding, cutting, and patterns in 2 and 3 dimensions
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