GATE 2025 Biochemistry Syllabus (XL-Q)

GATE 2025 syllabus for Biochemistry (XL-Q) gives you details of the latest GATE syllabus for the subject released by the official gate organizing institute IIT Roorkee 2025. We also created an easy-to-use ad-free mobile app for the GATE syllabus, previous year papers with keys, gate calculator, virtual calculator, and more. Download iStudy App for all GATE preparation needs.

You will also find IIT GATE college predictor, NIT GATE college predictor, GATE score calculator, and GATE Exam Info & Stats useful during GATE preparation. Without further due let’s jump into the GATE syllabus for Biochemistry.

GATE 2025 Syllabus – Biochemistry (XL-Q)

Section 1

Organization of life
Importance of water; Structure and function of biomolecules: Amino acids, Carbohydrates, Lipids, Proteins and Nucleic acids; Protein structure, folding / misfolding and function; Myoglobin, Hemoglobin, Lysozyme, Ribonuclease A, Carboxypeptidase and Chymotrypsin.

If you are preparing for GATE, download iStudy Mobile App for all GATE Syllabus, Previous Question Papers and Keys, preparation guide, Exam updates, Gate Virtual calculator, Gate Score calculator, and IITs & NITs cutoffs. It is a lightweight, easy to use, no images, and no ads platform to make students’ lives easier.
.

Section 2

Enzyme kinetics, regulation and inhibition
Vitamins and Coenzymes; Bioenergetics and metabolism; Generation and utilization of ATP; Metabolic pathways and their regulation: glycolysis, TCA cycle, pentose phosphate pathway, oxidative phosphorylation, gluconeogenesis, glycogen and fatty acid metabolism; Metabolism of Nitrogen containing compounds: nitrogen fixation, amino acids and nucleotides. Photosynthesis, Calvin cycle.

Section 3

Biochemical separation techniques
ion exchange, size exclusion and affinity chromatography, centrifugation; Characterization of biomolecules by electrophoresis; DNA- protein and protein – protein interactions; UV-visible and fluorescence spectroscopy; Mass spectrometry.

Section 4

Cell structure and organelles
Biological membranes; Action potential; Transport across membranes; Membrane assembly and Protein targeting; Signal transduction; Receptor-ligand interaction; Hormones and neurotransmitters.

Section 5

DNA replication, transcription and translation
DNA damage and repair; Biochemical regulation of gene expression; Recombinant DNA technology and applications: PCR, site directed mutagenesis, DNA-microarray; Next generation sequencing; Gene silencing and editing.

Section 6

Immune system
Innate and adaptive; Cell of the immune system; Active and passive immunity; Complement system; Antibody structure, function and diversity; B cell and T Cell receptors; B cell and T cell activation; Major histocompatibilty complex; Immunological techniques: Immunodiffusion, immune-electrophoresis, RIA and ELISA, flow cytometry; monoclonal antibodies and their applications.

If you are preparing for GATE, download iStudy Mobile App for all GATE Syllabus, Previous Question Papers and Keys, preparation guide, Exam updates, Gate Virtual calculator, Gate Score calculator, and IITs & NITs cutoffs. It is a lightweight, easy to use, no images, and no ads platform to make students’ lives easier.
.

GENERAL APTITUDE (Common for all branches)

Verbal Aptitude

Basic English Grammar
Tenses, articles, adjectives, prepositions, conjunctions, verb-noun agreement, and other parts of speech

Basic Vocabulary
Words, idioms, and phrases in context

Reading and Comprehension

Narrative Sequencing

Quantitative Aptitude

Data Interpretation
Data graphs (bar graphs, pie charts, and other graphs representing data), 2- and 3-dimensional plots, maps, and tables

Numerical Computation and Estimation
Ratios, percentages, powers, exponents and logarithms, permutations and combinations, and series

Mensuration and Geometry

Elementary Statistics and Probability

Analytical Aptitude

Logic
Deduction and Induction

Analogy

Numerical Relations and Reasoning

Spatial Aptitude

Transformation of Shapes
Translation, rotation, scaling, mirroring, assembling, and grouping

Paper folding, cutting, and patterns in 2 and 3 dimensions

InI India has tons of information for M.Tech Admissions in IITs, NITs, IIITs, and other state-level institutes. Do benefit from the content and also share it with your friends. If you want to discuss things with friends do check our communities, All Q&A about GATE Preparation and M.Tech Admissions.

We wish you great luck with GATE preparations.